By Tong-Cun Zhang, Motowo Nakajima

At the ICAB 2014, researchers from all over the world will assemble to debate the most recent clinical examine, findings and applied sciences referring to Microbial Genetics and Breeding, Optimization and keep an eye on of organic methods, organic Separation and organic Purification, and Advances in Biotechnology.
This convention will supply a platform for educational alternate at the program of biotechnology among household and foreign universities, learn institutes, company specialists and students. The contributors will concentrate on the overseas improvement and destiny tendencies. the development will lay an exceptional origin for addressing key technical demanding situations in a variety of components of utilized biotechnology, delivering possibilities to advertise the advance and enlargement of the biotechnology industry.

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Svasti J, Phongsak T, Sarnthima R (2003) Transglycosylation of tertiary alcohols using cassava β-glucosidase. Biochem Biophys Res Commun 305:470–475 8. Yan Q, Hua C, Yang S et al (2012) High level expression of extracellular secretion of β-glucosidase gene (PtBglu3) from Paecilomyces thermophila in Pichia pastoris. Protein Expr Purif 84:64–72 9. Ruanglek V, Sriprang R, Ratanaphan N et al (2007) Cloning, expression, characterization, and high cell-density production of recombinant endo-1,4-β-xylanase from Aspergillus niger in Pichia pastoris.

In our previous work, the wild irrE gene from D. radiodurans R1 has been heterologously expressed in E. coli BL21(DE3). The results showed that the growth rate and the final biomass of recombinant strain containing pET-28a(+)-irrE(W) under stress were significantly higher than the control strain (harboring the empty vector pET-28a(+)). In order to obtain the mutants with higher tolerance to stress, IrrE was further directed evolved by error-prone PCR using pET-28a(+)-irrE(W) as a template in this paper.

Gels were stained with ethidium bromide and visualized under UV in a transilluminator. PCR products were sent for sequencing (Genewiz, Beijing, China). 81. cgi). 05 and the Kimura 2-parameter distance nucleotide model. The tree was evaluated by 1,000 bootstrap replications. 0 for Windows. The results were expressed as means and standard deviations (±SD). 1 Spatial and Temporal Distribution of Microcystis novacekii The average biomass of M. 55 × 104 ind/L) in October, Fig. 03 × 104 ind/L) at Center West.

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