By Ingalis A.G. (ed.)

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Try reducing the SW by ca. 1 ppm and retry the acquisition. 8. , 51 as given in step 1; alternatively use the browser window and the drag-and-drop function to load the reference spectrum data folder into the spectrum window), compare the current spectrum with the reference. Look for peak-shape changes due to the decoupling. 32 Practical NMR Spectroscopy Laboratory Guide: Using Bruker Spectrometers 9. Copy this experiment to a new expno. , decouple a different peak), and check the decoupled spectrum as above.

9. After the experiment has completed, type rser 1 to read the first FID (of 8). In the new window, do ef and then phase this spectrum with all peaks inverted (this spectrum has the shortest VD). s2d which will save the phase corrections to the T1-IR (2D) dataset. Note: You also could read the last FID (#8) and phase its spectrum positive (normal fashion) and save these corrections. 10. Type to2d to return to the original 2D dataset. 11. Process the data using xf2 (Fourier transform in F2—the acquisition dimension).

Type slice then select “Rows & Columns” then click “Interactive row/column display” and “OK,” then click the “Scan rows” (z) button and move the mouse pointer to look at the 1D spectra that make up the dataset. Click the “return” icon (↵) in the icon bar to exit this mode. 16. 2 for an illustration of the relaxation calculation window): a. Click “Extract Slice” from the NMR Relaxation Guide (righthand side) pane, then in the pop-up window select “Spectrum” then enter 8 for the “Slice Number5” (to pick the last spectrum of the dataset) followed by “OK” b.

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